isolation of phosphate solubilizing microorganisms from wheat rhizosphere and evaluation of the their solubilization potential at two insoluble phosphate sources

نویسندگان

راضیه ابراهیمی کریم آباد

دانشگاه ارومیه-علوم خاک میرحسن رسولی صدقیانی

دانشگاه ارومیه محسن برین

دانشگاه ارومیه-علوم خاک

چکیده

phosphorus (p) is one a nutrient elements for plant growth. in most soils has complex behavior and forms sparingly soluble compounds with soil particles. phosphorus bioavailability can be controlled by soil biological activities for optimum nutrition of plants. this study was carried out in order to isolation of p-solubilizing bacteria (psb) as well as p-solubilizing fungi (psf) and their screening in terms of p solubilizing potential in presence of tricalcium phosphate (tcp) and rock phosphate (rp). a total of 55 soil samples were taken from the wheat rhizosphere of urmia region, and the isolation as well as qualitative solubilizing potential was done in solid nbrip media. quantitative p-dissolution ability of isolated strains were assessed in a factorial experiment as completely randomized design consisting phosphate sources (rp and tcp) and slected microbial isolates (control, psb45, psb15, psb30, psb2, psb11, psb12, psb20, psf1, psf3, psf4 and psf7) in seven incubation times (0, 1, 3, 6, 9, 12 and 15 days). the results showed that p solubilization and medium ph significantly (p

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عنوان ژورنال:
تحقیقات کاربردی خاک

جلد ۳، شماره ۲، صفحات ۲۹-۴۱

کلمات کلیدی
[ ' p h o s p h o r u s ( p ) i s o n e a n u t r i e n t e l e m e n t s f o r p l a n t g r o w t h . i n m o s t s o i l s h a s c o m p l e x b e h a v i o r a n d f o r m s s p a r i n g l y s o l u b l e c o m p o u n d s w i t h s o i l p a r t i c l e s . p h o s p h o r u s b i o a v a i l a b i l i t y c a n b e c o n t r o l l e d b y s o i l b i o l o g i c a l a c t i v i t i e s f o r o p t i m u m n u t r i t i o n o f p l a n t s . t h i s s t u d y w a s c a r r i e d o u t i n o r d e r t o i s o l a t i o n o f p ' , ' s o l u b i l i z i n g b a c t e r i a ( p s b ) a s w e l l a s p ' , ' s o l u b i l i z i n g f u n g i ( p s f ) a n d t h e i r s c r e e n i n g i n t e r m s o f p s o l u b i l i z i n g p o t e n t i a l i n p r e s e n c e o f t r i c a l c i u m p h o s p h a t e ( t c p ) a n d r o c k p h o s p h a t e ( r p ) . a t o t a l o f 5 5 s o i l s a m p l e s w e r e t a k e n f r o m t h e w h e a t r h i z o s p h e r e o f u r m i a r e g i o n ' , ' a n d t h e i s o l a t i o n a s w e l l a s q u a l i t a t i v e s o l u b i l i z i n g p o t e n t i a l w a s d o n e i n s o l i d n b r i p m e d i a . q u a n t i t a t i v e p ' , ' d i s s o l u t i o n a b i l i t y o f i s o l a t e d s t r a i n s w e r e a s s e s s e d i n a f a c t o r i a l e x p e r i m e n t a s c o m p l e t e l y r a n d o m i z e d d e s i g n c o n s i s t i n g p h o s p h a t e s o u r c e s ( r p a n d t c p ) a n d s l e c t e d m i c r o b i a l i s o l a t e s ( c o n t r o l ' , ' p s b 4 5 ' , ' p s b 1 5 ' , ' p s b 3 0 ' , ' p s b 2 ' , ' p s b 1 1 ' , ' p s b 1 2 ' , ' p s b 2 0 ' , ' p s f 1 ' , ' p s f 3 ' , ' p s f 4 a n d p s f 7 ) i n s e v e n i n c u b a t i o n t i m e s ( 0 ' , 1 , 3 , 6 , 9 , ' 1 2 a n d 1 5 d a y s ) . t h e r e s u l t s s h o w e d t h a t p s o l u b i l i z a t i o n a n d m e d i u m p h s i g n i f i c a n t l y ( p ' ]

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